Argentina internationals Sergio Romero, Marcos Rojo and England international Phil Jones all being left out, from Ole Gunnar Solskjaer’s list.
United who were drawn in group H, alongside with French Champions Paris Saint Germain, RB Leipzig and Istanbul Basaksehir.
New signings, Edinson Cavani, Alex Telles and Donny van de Beek all made the cut in the 25 man list.
The Argentine goalkeeper saw his spot given to Dean Henderson, who was loaned out to Sheffield United last season, but returned back this season.
Five substitutions will be permitted in each Champions League game this season, which means an extended 23-man matchday squad can be named.
Man Utd A-list squad
Goalkeepers: David de Gea, Dean Henderson, Lee Grant
Defenders: Eric Bailly, Timothy Fosu-Mensah, Victor Lindelof, Harry Maguire, Luke Shaw, Alex Telles, Axel Tuanzebe, Aaron Wan-Bissaka
Midfielders: Bruno Fernandes, Fred, Daniel James, Jesse Lingard, Juan Mata, Nemanja Matic, Scott McTominay, Facundo Pellistri, Paul Pogba, Donny van de Beek
Forwards: Edinson Cavani, Odion Ighalo, Anthony Martial, Marcus Rashford